4000-520-616
欢迎来到免疫在线！(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
产品库 品牌馆 现货促销 技术交流 新闻动态 联系我们
novusbio
主营：主营：分子类，蛋白类，抗体类，生化类试剂
咨询热线电话
4000-520-616
公司首页 公司介绍 产品中心 新闻动态 联系方式 在线下单
当前位置： 首页 > 产品中心 > Cell_lysis > Novus/Recombinant Human TGF-beta 1 Protein/10 ug/240-B-010
商品详细Novus/Recombinant Human TGF-beta 1 Protein/10 ug/240-B-010
Novus/Recombinant Human TGF-beta 1 Protein/10 ug/240-B-010
Novus/Recombinant Human TGF-beta 1 Protein/10 ug/240-B-010
商品编号： 240-B-010
品牌： novusbio
市场价： ¥16180.00
美元价： 9708.00
产地： 美国(厂家直采)
公司：
产品分类： 细胞裂解和提取物
公司分类： Cell_lysis
联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
立即询价
商品介绍

RecombinantHumanTGF-beta1ProteinSummary

AdditionalInformation
ANewTGF-beta1isAvailable!ThenewproteinisHek293expressed!
DetailsofFunctionality
MeasuredbyitsABIlitytoinhibittheIL-4-dependentproliferationofHT‑2mouseT cells.Tsang, M.et al.(1995)Cytokine7:389.TheED50forthiseffectis0.04-0.2ng/mL.ThespecificactivityofRecombinantHumanTGF-beta1isapproximately2.5x104U/μg,whichiscalibratedagainsthumanTGF‑beta1Standard(NIBSCcode:89/514).
Source
ChineseHamsterOvarycellline,CHO-derivedhumanTGF-beta1proteinAla279-Ser390
Accession#
P01137
N-terminalSequence
Ala279
Structure/Form
Disulfide-linkedhomodimer
Protein/PeptideType
RecombinantProteins
Gene
TGFB1
Purity
>97%,bySDS-PAGEvisualizedwithSilverStainingandquantitativedensitometrybyCoomassie®BlueStaining.
EndotoxinNote
<0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Applications/Dilutions

TheoreticalMW
12.8kDa(monomer).Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
SDS-PAGE
12kDa,reducingconditions24kDa,non-reducingconditions
Publications
ReadPublicationsusing240-Binthefollowingapplications:
  • Bioassay
    570publications
  • CellCulture
    9publications
  • ELISA(Standard)
    7publications
  • ELISADevelopmet
    1publication
  • ELISAStandard
    1publication
  • InVivo
    16publications
  • Standard
    1publication
  • SurfacePlasmonResonance(SPR
    1publication
  • WesternBlot
    4publications

Packaging,Storage&Formulations

Storage
Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
  • 12monthsfromdateofreceipt,-20to-70°Cassupplied.
  • 1month,2to8°Cundersterileconditionsafterreconstitution.
  • 3months,-20to-70°Cundersterileconditionsafterreconstitution.
Buffer
Lyophilizedfroma0.2μmfilteredsolutioninAcetonitrileandTFAwithBSAasacarrierprotein.
Purity
>97%,bySDS-PAGEvisualizedwithSilverStainingandquantitativedensitometrybyCoomassie®BlueStaining.
ReconstitutionInstructions
PurifiedrecombinanthumanTGF-beta1isanextremelyhydrophobicproteinthatadheresstronglytosurfaces.Toensurerecovery,reconstituteat20μg/mLinsterile4mMHClcontaining1mg/mLhumanorbovineserumalbumin.

Notes

ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.

AlternateNamesforRecombinantHumanTGF-beta1Protein

  • CEDLAP
  • DPD1
  • latency-associatedpeptide
  • TGFbeta1
  • TGFB
  • TGFB1
  • TGF-beta1protein
  • TGFbeta1
  • TGF-beta1
  • TGFbeta
  • TGF-beta-1
  • transforminggrowthfactorbeta-1
  • transforminggrowthfactor,beta1

Background

TGF-beta1(transforminggrowthfactorbeta1)isoneofthreecloselyrelatedmammalianmembersofthelargeTGF-betasuperfamilythatshareacharacteristiccystineknotstructure(1‑7).TGF-beta1,-2and-3arehighlypleiotropiccytokinesthatareproposedtoactascellularswitchesthatregulateprocessessuchasimmunefunction,proliferationandepithelial-mesenchymaltransition(1‑4).EachTGF-betaisoformhassomenon‑redundantfunctions;forTGF-beta1,micewithtargeteddeletionshowdefectsinhematopoiesisandendothelialdifferentiation,anddieofoverwhelminginflammation(2).HumanTGF‑beta1CDNAencodesa390aminoacid(aa)precursorthatcontainsa29aasignalpeptideanda361aaproprotein(8).Afurin‑likeconvertaseprocessestheproproteintogenerateanN‑terminal249aalatency‑associatedpeptide(LAP)andaC‑terminal112aamatureTGF‑beta1(8,9).Disulfide‑linkedhomodimersofLAPandTGF‑beta1remainnon‑covalentlyassociatedaftersecretion,formingthesmalllatentTGF‑beta1complex(8‑10).CovalentlinkageofLAPtooneofthreelatentTGF‑betabindingproteins(LTBPs)createsalargelatentcomplexthatmayinteractwiththeextracellularmatrix(9,10).TGF‑betaisactivatedfromlatencybypathwaysthatincludeactionsoftheproteaseplasmin,matrixmetalloproteases,thrombospondin1andasubsetofintegrins(10).MaturehumanTGF‑beta1shares100%aaidentitywithpig,dogandcowTGF‑beta1,and99%aaidentitywithmouse,ratandhorseTGF‑beta1.Itdemonstratescross‑speciesactivity(1).TGF‑beta1signalingbeginswithhigh‑affinitybindingtoatypeIIser/thrkinasereceptortermedTGF‑betaRII.Thisreceptorthenphosphorylatesandactivatesasecondser/thrkinasereceptor,TGF‑betaRI(alsocalledactivinreceptor‑likekinase(ALK)‑5),oralternatively,ALK‑1.ThiscomplexphosphorylatesandactivatesSmadproteinsthatregulatetranscription(3,11,12).Contributionsoftheaccessoryreceptorsbetaglycan(alsoknownasTGF‑betaRIII)andendoglin,oruseofSmad‑independentsignalingpathways,allowfordisparateactionsobservedinresponsetoTGF‑betaindifferentcontexts(11).
  1. Derynck,R.andK.Miyazono(2008)ColdSpringHarborLaboratoryPress,29.
  2. Dunker,N.andK.Krieglstein(2000)Eur.J.Biochem.267:6982.
  3. Wahl,S.M.(2006)Immunol.Rev.213:213.
  4. Chang,H.etal.(2002)Endocr.Rev.23:787.
  5. Lin,J.S.etal.(2006)Reproduction132:179.
  6. Hinck,A.P.etal.(1996)Biochemistry35:8517.
  7. Mittl,P.R.E.etal.(1996)ProteinSci.5:1261.
  8. Derynck,R.etal.(1985)Nature316:701.
  9. Miyazono,K.etal.(1988)J.Biol.Chem.263:6407.
  10. Oklu,R.andR.Hesketh(2000)Biochem.J.352:601.
  11. deCaestecker,M.etal.(2004)CytokineGrowthFactorRev.15:1.
  12. Zuniga,J.E.etal.(2005)J.Mol.Biol.354:1052.

CustomersWhoViewedThisItemAlsoViewed...

TNF-alpha[Unconjugated]
210-TA
Recombinant Human TNF-alpha(Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100&amp;nbsp;pg/mL.
1 µg/lane of Recombinant Human TNF-alphawas resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(24Reviews)
(607Publications)
Species:Hu
IL-6[Unconjugated]
7270-IL/CF
IL-6 [Unconjugated]
Species:Hu
Smad2/3Antibody[Unconjugated]
AF3797
Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed&amp;nbsp;with 0.5&amp;nbsp;µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). For additional reference, recombinant human Smad2 and Smad3 were included. Specific bands for Smad2 were detected at approximately 64 and 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group&amp;nbsp;2.
Simple Western lane view shows lysates of A549 human lungcarcinoma cell line, COLO&amp;nbsp;205 human colorectal adenocarcinoma cellline, and HT‑29 human colon adenocarcinoma cell line, loaded at0.2&amp;nbsp;mg/mL. A specific band was detected for Smad2/3 at approximately64&amp;nbsp;kDa (as indicated) using 25&amp;nbsp;µg/mL of GoatAnti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody(Catalog # AF3797) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgGSecondary Antibody (Catalog&amp;nbsp;# HAF109).This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog&amp;nbsp;# AB‑108‑C) for 15&amp;nbsp;minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog&amp;nbsp;# MAG999) and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR.
(2Reviews)
(12Publications)
Species:Hu,Mu
Applications:WB,SimpleWestern,ChIP,ICC
Smad3Antibody
NB100-56479
Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show aband around 55 Kda. 1:1000 dilution, 1 hour blocking in milk 5%. Image from a verified customer review.
Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Smad3 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/ml.
(1Review)
(2Publications)
Species:Hu,Mu,Rt,Po,Bv,Ca,Pm
Applications:WB,ICC/IF,IHC,IHC-P
CTGF/CCN2Antibody
NB100-724
Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
(4Publications)
Species:Hu,Mu,Rt
Applications:WB,ICC/IF,IHC,IHC-P
FibronectinAntibody
NBP1-91258
Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate.
Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.
(9Reviews)
(23Publications)
Species:Hu,Mu,Rt,Po,Bv,Ca,Eq,Fe
Applications:WB,SimpleWestern,ICC/IF,IHC,IHC-Fr,IHC-P
FGFbasic/FGF2/bFGF[Unco
233-FB
Recombinant Human FGF basic/FGF2/bFGF (146 aa) (Catalog # 233-FB) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The activity is approximately 3-fold greater than the top competitor
1 µg/lane of Recombinant Human&amp;nbsp;FGF basic/FGF2/bFGF (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(13Reviews)
(340Publications)
Species:Hu
LAP3Antibody
NBP1-82847
Western Blot: LAP3 Antibody [NBP1-82847] - Analysis using Anti-LAP3 antibody NBP1-82847 (A) shows similar pattern to independent antibody NBP1-82848 (B).
Immunocytochemistry/Immunofluorescence: LAP3 Antibody [NBP1-82847] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol &amp; midbody.
Immunohistochemistry-Paraffin: LAP3 Antibody [NBP1-82847] - Staining of human kidney.
Species:Hu
Applications:WB,ICC/IF,IHC,IHC-P
TGF-betaRI/ALK-5Antibody(141231)[Un...
MAB5871
TGF-beta RI/ALK-5 Antibody (141231) [Unconjugated]
(1Review)
(5Publications)
Species:Hu,Mu
Applications:WB
EGF[Unconjugated]
236-EG
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.
1 µg/lane of Recombinant Human EGF was resolved&amp;nbsp;with SDS-PAGE&amp;nbsp; and visualized&amp;nbsp;by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
(14Reviews)
(413Publications)
Species:Hu
Smad4Antibody(SP05-05)
NBP2-67372
Western Blot: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of Smad4 on PC-12 cells lysates using anti-Smad4 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Smad4 Antibody (SP05-05) [NBP2-67372] - Staining Smad4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Smad4 Antibody (SP05-05) [NBP2-67372] - Analysis of paraffin-embedded mouse colon tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Species:Hu,Mu,Rt
Applications:WB,Flow,ICC/IF,IHC,IHC-P
TGF-beta1[Unconjugated]
7754-BH/CF
TGF-beta 1 [Unconjugated]
(4Publications)
Species:Hu
ERK2Antibody(SZ25-01)
NBP2-67360
Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Species:Hu,Mu,Rt
Applications:WB,Flow,ICC/IF,IHC,IHC-P,IP
IL-4
6507-IL/CF
IL-4
(3Publications)
Species:Hu
Smad7Antibody(293039)[Unconjugate
MAB2029
Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1&amp;nbsp;µg/mL of Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog&amp;nbsp;# HAF007). A specific band was detected for Smad7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Mouse HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of Sf&amp;nbsp;21 S.frugiperda insect ovarian cell line transfected with human Smad7, loaded at0.2&amp;nbsp;mg/mL. A specific band was detected for Smad7 at approximately56&amp;nbsp;kDa (as indicated) using 10&amp;nbsp;µg/mL of MouseAnti-Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029). Thisexperiment was conducted under reducing conditions and using the 12-230 kDaseparation system.
(1Review)
(23Publications)
Species:Hu,Mu,Rt
Applications:WB,SimpleWestern,IHC
RecombinantHumanTGF-beta1Protein
240-B
Recombinant Human TGF-beta 1 Protein
Species:Hu
Applications:Bioactivity
品牌介绍
  点击授权经销商名单，订购品质保障产品。   Novus抗体   Novus蛋白   Valukine™ ELISA试剂盒 33,000+产品引用文献 3,000+产品客户五星推荐 200,000产品应用数据 9,000+小包装抗体   点击查看抗体产品 高生物活性 高纯度 超低内毒素水平 批次之间非常稳定   点击查看蛋白产品 产品源自美国 专供中国市场 保证准确性、灵敏度、重复性和特异性 点击查看Valukine™ ELISA产品   Novus Biologicals，抗体一站式服务专家。 作为世界上最大的抗体生产商，Novus Biologicals 携近16万种抗体席卷而来，完美解决您的需求。 奖励计划 Novus产品评价奖励计划：即日起，Novus奖励计划将不需要累积积分，即可直接获得亚马逊礼物卡。具体新计划详情请点击此海报。 文献奖励计划：您使用Novus的产品发表过文章吗？把您的发表信息提供给我们，即可换取价值￥300或￥600的亚马逊卡。 奖励计划中文细则，请点击此处。   创新奖励计划，你验证，我买单 提交所购买一抗尚未验证的物种或应用数据，奖励所购买一抗价格等值抵用券 创新奖励计划，如何获得抵用券 向我司授权代理商索取创新奖励抗体申请表 填写图片、实验步骤和结果说明等信息，提交到techsupport.cn@bio-techne.com 核实图片、实验步骤和结果说明 信息确认后，我司通过邮件发送与您所购买产品价格等值抵用券 抵用券仅适用于Novus Biologicals的一抗产品，具体细则，详见抵用券上的说明 注：参加创新奖励，请登录Novus官网一抗页面，在species 和tested application选项确认验证的物种或应用不在列表中
公司介绍
  点击授权经销商名单，订购品质保障产品。   Novus抗体   Novus蛋白
品牌分类
Inhibitors 组织微阵列 样本量抗体 工具箱 Antibody Packs Peptides and Proteins Lysates 蛋白质印迹膜 Support Products
显示分类
联络我们
服务热线：4000-520-616
（限工作日9:00-18:00）
QQ ：1570468124
手机：18915418616
快速导航：
总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
工作时间：周一至周五9:00~18:00
技术支持：
短信：18915418616
服务热线：
4000-520-616（全国）
☏  0512-67156496（苏州）
版权信息：
©  2015-2022 苏州蚂蚁淘生物科技有限公司 版权所有 中国
网站备案：
友情链接：
免责声明：
本站有部分内容来自互联网，如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权，请来电或致函告之，
本网站将在规定时间内给予删除等相关处理。
扫描二维码关注官方微信
获取最新促销及产品信息