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当前位置: 首页 > 产品中心 > Functional_antibody > Novus/INSTA-Blot人脑/NBP2-30119
商品详细Novus/INSTA-Blot人脑/NBP2-30119
Novus/INSTA-Blot人脑/NBP2-30119
Novus/INSTA-Blot人脑/NBP2-30119
商品编号: NBP2-30119
品牌: novusbio
市场价: ¥7980.00
美元价: 4788.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description
Ready-to-use PVDF membrane with human brain lysate (30 ug total protein per lane).
Background
INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Human Brain NBP2-30119 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We"ve streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With IN...STA-Blot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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PreparationMethod
The INSTA-Blot™ Human Brain NBP2-30119 contains denatured proteins from tissue lysates loaded at 30 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is provided ready-to-use in your western blot protocol.
Application Notes
Human tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in Western blot reported in scientific literature (PMID 26198933)
Publications
ReadPublication using NBP2-30119.
品牌介绍
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