Summary:

Immunogen	ApeptidederivedfromtheC-terminusofmouse/humanHIF-2alphaprotein.
Specificity	SpecificforHIF-2alpha/EPAS.Doesnotcross-reactwithHIF-1alpha.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	EPAS1
Purity	Immunogenaffinitypurified
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions	WesternBlot1.0ug/ml-2.0ug/ml SimpleWestern1:50 ChromatinImmunoprecipitation1:10-1:500 ELISA1:100-1:2000 FlowCytometry GelSuperShiftAssays Immunoblotting Immunocytochemistry/Immunofluorescence1:100 Immunohistochemistry1:100 Immunohistochemistry-Frozen Immunohistochemistry-Paraffin1:100 Immunoprecipitation5ug/1mglysate
ApplicationNotes	ThisHIF-2alphaantibodyisusefulforWesternblot,Immunoprecipitation,ChromatinImmunoprecipitation,ELISA,GelShiftSuperAssay(PMID:15184875),UseinImmunoblottingreportedinscientificliterature(PMID28115701)..Immunocytochemistry/ImmunofluorescenceandImmunohistochemistryonparaffin-embeddedsections.InWB,thisantibodyrecognizesabandat118kDarepresentingHIF-2alpha. InSimpleWesternonly10-15uLoftherecommendeddilutionisusedperdatapoint.SeparatedbySize-Wes,SallySue/PeggySue. Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
TheoreticalMW	118kDa. Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
PositiveControl	PC-12Lysate(NB810-55229) COS-7Lysate(NB800-PC26) RectumLysate(NB820-59250)
ReviewedApplications	Read28Reviewsrated4.4 using NB100-122inthefollowingapplications: WesternBlot ChromatinImmunoprecipitation Immunohistochemistry-Paraffin Immunofluorescence
Publications	ReadPublicationsusing NB100-122inthefollowingapplications: ChIP 38publications Co-IP 1publication ELISA 5publications FLOW 3publications Flow 1publication GS 2publications IB 20publications ICC/IF 18publications IHC 21publications IHC-Fr 4publications IHC-P 28publications IP 16publications WB 319publications

ReactivityNotes:

Reactivitywithsheepreportedinthescientificliterature(PMID:17307811).Hamsterreactivityreportedinscientificliterature(PMID:24997364and24997360)

Packaging,Storage&Formulations:

Storage	Storeat-20°C.
Buffer	PBS
Preservative	0.05%SodiumAzide
Concentration	1.0mg/ml
Purity	Immunogenaffinitypurified

Notes:

• antihif2alpha
• anti-HIF-2alpha
• Basic-helix-loop-helix-PASproteinMOP2
• BHLHE73
• bHLHe73ECYT4
• ClassEbasichelix-loop-helixprotein73
• endothelialPASdomainprotein1
• endothelialPASdomain-containingprotein1
• EPAS1
• EPAS-1
• HIF2A
• HIF-1alpha-likefactor
• HIF2alpha
• HIF-2alpha
• hif2aangiogenesis
• HIF2A
• HIF2AHIF-2-alpha
• HLF
• HLFHIF-1-alpha-likefactor
• hypoxia-inducIBLefactor2alpha
• Hypoxia-induciblefactor2-alpha
• MemberofPASprotein2
• MOP2
• MOP2HIF2-alpha
• PASD2PASdomain-containingprotein2

AlternateNamesfor53BP1Antibody:

Hypoxia-induciblefactors(HIFs)generateheterodimerscomposedofalpha-subunits(HIF-1alpha,HIF-2alphaandHIF-3alpha)andbetasubunits(Arnt1,Arnt2andArnt3).Oftheseisoforms,HIF-1alphaandHIF-2alphaplaycriticalrolesinthecellularresponsetohypoxicconditions.AfterheterodimerizationwithbetasubunitsbindingtoDNAsequence5"-[AG]CGTG-3"withinHRE(hypoxiaresponseelement)oftargetgenepromoters,HIF-2alpharegulatesthegeneexpressioninresponsetooxygenhomeostasisalterations.HIF-2alpharegulatesVEGFexpressioninvolvedinthedevelopmentofnewbloodvesselsaswellasthetubularsystemofthelung.HIF-2alphaplaysaroleintheformationoftheendotheliumthatgivesrisetobloodbrainbarrier.HIF-2alphaisastrongactivatorofexpressionofTie-2tyrosinekinase,whichisamajorplayerinangiogenesis.HIF-2alphaactivationrequiresrecruitmentoftranscriptionalcoactivatorssuchasCREBPB,EP300,anditsinteractionwithredoxregulatoryproteinAPEXmediatesactivationofCTAD.HIF-2alphaandHIF-1alphaexpressionincreasesincertainmalignancies;wherein,HIF-1alphaplaysbothpro-aswellasanti-proliferativeactivities.HIF-2alphaissuggestedtoexertanti-proliferativeactivityonly.

Background:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.

Limitations:

Hypoxia-induciblefactors(HIFs)generateheterodimerscomposedofalpha-subunits(HIF-1alpha,HIF-2alphaandHIF-3alpha)andbetasubunits(Arnt1,Arnt2andArnt3).Oftheseisoforms,HIF-1alphaandHIF-2alphaplaycriticalrolesinthecellularresponsetohypoxicconditions.AfterheterodimerizationwithbetasubunitsbindingtoDNAsequence5"-[AG]CGTG-3"withinHRE(hypoxiaresponseelement)oftargetgenepromoters,HIF-2alpharegulatesthegeneexpressioninresponsetooxygenhomeostasisalterations.HIF-2alpharegulatesVEGFexpressioninvolvedinthedevelopmentofnewbloodvesselsaswellasthetubularsystemofthelung.HIF-2alphaplaysaroleintheformationoftheendotheliumthatgivesrisetobloodbrainbarrier.HIF-2alphaisastrongactivatorofexpressionofTie-2tyrosinekinase,whichisamajorplayerinangiogenesis.HIF-2alphaactivationrequiresrecruitmentoftranscriptionalcoactivatorssuchasCREBPB,EP300,anditsinteractionwithredoxregulatoryproteinAPEXmediatesactivationofCTAD.HIF-2alphaandHIF-1alphaexpressionincreasesincertainmalignancies;wherein,HIF-1alphaplaysbothpro-aswellasanti-proliferativeactivities.HIF-2alphaissuggestedtoexertanti-proliferativeactivityonly.

  点击授权经销商名单，订购品质保障产品。   Novus抗体   Novus蛋白   Valukine™ ELISA试剂盒 33,000+产品引用文献 3,000+产品客户五星推荐 200,000产品应用数据 9,000+小包装抗体   点击查看抗体产品 高生物活性 高纯度 超低内毒素水平 批次之间非常稳定   点击查看蛋白产品 产品源自美国 专供中国市场 保证准确性、灵敏度、重复性和特异性 点击查看Valukine™ ELISA产品   Novus Biologicals，抗体一站式服务专家。 作为世界上最大的抗体生产商，Novus Biologicals 携近16万种抗体席卷而来，完美解决您的需求。 奖励计划 Novus产品评价奖励计划：即日起，Novus奖励计划将不需要累积积分，即可直接获得亚马逊礼物卡。具体新计划详情请点击此海报。 文献奖励计划：您使用Novus的产品发表过文章吗？把您的发表信息提供给我们，即可换取价值￥300或￥600的亚马逊卡。 奖励计划中文细则，请点击此处。   创新奖励计划，你验证，我买单 提交所购买一抗尚未验证的物种或应用数据，奖励所购买一抗价格等值抵用券 创新奖励计划，如何获得抵用券 向我司授权代理商索取创新奖励抗体申请表 填写图片、实验步骤和结果说明等信息，提交到techsupport.cn@bio-techne.com 核实图片、实验步骤和结果说明 信息确认后，我司通过邮件发送与您所购买产品价格等值抵用券 抵用券仅适用于Novus Biologicals的一抗产品，具体细则，详见抵用券上的说明 注：参加创新奖励，请登录Novus官网一抗页面，在species 和tested application选项确认验证的物种或应用不在列表中