Sample size antibodies: Novus Biologicals

来自 : 发布时间：2024-05-06

Note: Mouseover a species abbreviation on the product page to display the fullname. Products (52733)Full Site page of 4395Sample-size AntibodiesThe following antibodies are supplied in a full-size and sample-size (trial) vial. To see the pricing and availability information on the sample size, please go to the datasheet and change the package size in the Ordering Information section. In addition, use the filters to the left and the sorting function to find an antibody of interest that is supplied as a sample size.52733 ResultsListGrid NB100-2220LC3B AntibodyPurity: Immunogen affinity purifiedImmunogen: Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8]Other Names: anti-LC3B, atg8f, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B\"> Genetic Strategies Validation. Knockout Validated: LC3B Antibody [NB100-2220] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.\" > Genetic Strategies Validation. Knockout Validated: LC3B Antibody [NB100-2220] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.\" > Biological Strategies Validation. Western Blot: LC3B Antibody [NB100-2220] - Lysates of mouse NIH3T3 and rat PC-12 cell lines untreated (-) or treated (+) with Chloroquine. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL rabbit anti-LC3B polyclonal Antibody (Catalog # NB100-2220, Novus Biologicals), followed by 1:2000 dilution of goat anti-rabbit IgG secondary antibody. LC3 detected at a molecular weight of approximately 15 kDa in treated NIH3T3 and PC-12 cells.\" > Biological Strategies Validation. Western Blot: LC3B Antibody [NB100-2220] - Analysis of LC3 in untreated and treated H9C2 cells using Rabbit anti-LC3B Antibody [Catalog # NB100-2220].\" > Biological Strategies Validation. Western Blot: LC3B Antibody [NB100-2220] - Detection of LC3I and LC3II in mouse cochlea cell line SV-K1 using Rabbit anti-LC3B Antibody [Catalog # NB100-2220]. Cells were treated with chloroquine (1 uM), and As2O3 (1 uM) for 24 hrs. Image from verified customer review.\" > Biological Strategies Validation. Western Blot: LC3B Antibody [NB100-2220] - Expression of autophagic protein LC3 in BME-UV1 cells forming acinar structures on Matrigel. Representative images of Western blot analysis of LC3 in bovine MECs grown in 3D culture for 3, 6, 9, and 14 days in differentiation medium (control), enriched with 17beta-estradiol (E2, 1 nM), progesterone (P4, 5 ng/mL), or both (E + P); expression of beta-actin was used as a loading control; graphs below the images show the results of densitometric analysis, in which IOD of each band was measured, and the values were normalized to IOD of beta-actin; the IOD results are presented as means +/- SEM from at least three separate experiments. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2014/382653/) licensed under a CC-BY licence.\" > Reactivity:Hu, Mu, Rt, Po, Al, Av, Ba, Bv, Ca, Ch, Gp, Ha, In, Pm, Pm, Rb, SyHa, ZeSpecies GlossaryReactivity:Hu, Mu, Rt, Po, Al, Av, Ba, Bv, Ca, Ch, Gp, Ha, In, Pm, Pm, Rb, SyHa, ZeApplications:WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, ChIP, KD, KOApplications:WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, ChIP, KD, KOHost:Rabbit PolyclonalHost:Rabbit PolyclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Validated by:Validated by: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB100-105HIF-1 alpha Antibody (H1alpha67)Purity: Protein G purifiedImmunogen: This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].Other Names: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, H1alpha67, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1, PAS domain-containing protein 8, PASD8\"> Biological Strategies Validation. Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. WB image submitted by a verified customer review.\" > Biological Strategies Validation and Genetic Strategies Validation.Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog # NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.\" > Biological Strategies Validation and Genetic Strategies Validation.Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review.\" > Biological Strategies Validation. Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis of HIF-1 alpha in human hepatocytes from cancer patient using anti-HIF-1 alpha antibody NB100-105. Western blot image submitted by a verified customer review.\" > Biological Strategies Validation. Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50 ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.\" > Reactivity:Hu, Mu, Rt, Po, Bv, Ca, Fe, Ma, Pm, Pm, Rb, Sh, XpSpecies GlossaryReactivity:Hu, Mu, Rt, Po, Bv, Ca, Fe, Ma, Pm, Pm, Rb, Sh, XpApplications:WB, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, LA, PLA, TCS, IHC-FrFl, KD, KOApplications:WB, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, LA, PLA, TCS, IHC-FrFl, KD, KOHost:Mouse MonoclonalHost:Mouse MonoclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Validated by:Validated by: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB100-122HIF-2 alpha/EPAS1 AntibodyPurity: Immunogen affinity purifiedImmunogen: This HIF-2 alpha/EPAS1 Antibody was developed against a peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.Other Names: anti hif 2 alpha, anti-HIF-2 alpha, Basic-helix-loop-helix-PAS protein MOP2, BHLHE73, Class E basic helix-loop-helix protein 73, ECYT4, endothelial PAS domain protein 1, endothelial PAS domain-containing protein 1, EPAS1, EPAS-1, HIF-1-alpha-like factor, HIF-1alpha-like factor, HIF-2 alpha, HIF2A, hif2a angiogenesis, HIF-2-alpha, HIF2-alpha, HLF, hypoxia-inducible factor 2 alpha, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, MOP2, PAS domain-containing protein 2, PASD2\"> Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types. Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY licence.\" > Biological Strategies Validation. Knockout Validated: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF-1alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia. EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. Non-infected cells and GFP infected cells served as controls. Immunoblot analyses of EMSCs derived from the indicated genotypes treated with 0.1 mM DMOG for 24 hrs. Attenuated WNT11 expression in Hif-1alpha KO EMSCs (lenti-Cre infected Hif-1af/f). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY licence.\" > Orthogonal Strategies Validation. Dual RNAscope ISH-IHC: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Formalin-fixed paraffin-embedded tissue sections of human placenta were probed for HIF-2 alpha/EPAS1 mRNA (ACD RNAScope Probe, catalog #410598; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB100-122) at 1:100 dilution with one-hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to trophoblastic cells.\" > Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF-2 alpha in human retinal and choroidal primary endothelia lysates using.. Image from verified customer review.\" > Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF-2 alpha in MDA-MB-231 cell lysate (overexpression and endogenous samples) using. did not react to HIF-1 alpha overexpression. Image from verified customer review.\" > Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Lane 1: Cobalt chloride treated COS7 nuclear extracts. Lane 2: Untreated COS7 nuclear extracts.\" > Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis using the HRP conjugate of NB100-122. Detection of normoxic and hypoxic nuclear rat cell lysates.\" > Genetic Strategies Validation. Knockdown Validated: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Reduction of HIF-2alpha levels leads to protection in UV-triggered apoptosis, but not for apoptosis caused by glucose and serum starvation in 786-O cells. Parental 786-O or those either stably expressing wild-type VHLp19 or stably infected with a control vector (pSuperRetro) or a pool of two HIF-2alpha shRNAs vectors [21] were grown to confluence and lysed. Cell alpha-tubulin.\" > Reactivity:Hu, Mu, Rt, Fi, Ha, Pm, Rb, Re, ShSpecies GlossaryReactivity:Hu, Mu, Rt, Fi, Ha, Pm, Rb, Re, ShApplications:WB, Simple Western, ChIP, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, PAGE, Dual ISH-IHC, KD, KOApplications:WB, Simple Western, ChIP, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, PAGE, Dual ISH-IHC, KD, KOHost:Rabbit PolyclonalHost:Rabbit PolyclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Validated by:Validated by: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB100-30453BP1 AntibodyPurity: Immunogen affinity purifiedImmunogen: The epitope recognized by 53BP1 Antibody maps to a region between residues 350 and 400 of human 53BP1 [NP_005648.1].Other Names: 53BP1, p202, p53-binding protein 1, p53bp1, TDRD30, TP53-Binding Protein 1, tumor protein 53-binding protein, 1, tumor protein p53 binding protein 1, tumor protein p53-binding protein, 1, tumor suppressor p53-binding protein 1\"> Genetic Strategies Validation. Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.\" > Biological Strategies Validation. Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Effect of RK-33 on radiation-induced DNA damage. A Immunofluorescence images showing 53BP1 and gamma-H2AX foci in A549 cells after 2-Gy radiation and A549 cells pre-treated with 6 uM RK-33, 12 h before radiation. Overlap of 53BP1 and gamma-H2AX is seen in the merged picture of the co-immunofluorescence staining. Scale bar is 2 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.15252/emmm.201404368) licensed under a CC-BY licence.\" > Biological Strategies Validation. Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: 53BP1 foci in proliferating MEFs. Lower Panel: 53BP1 foci in proliferating MEFs exposed to 10 Gy of IR.\" > Biological Strategies Validation. Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Upper Panel: Control untreated cells. Lower Panel: Cells exposed to irradiation (10 Gy) and probed for 53BP1 foci. Cells were grown on coverslips, fixed with 4% paraformaldehyde, methanol permeabilized, blocked for 1 h, RT. Incubated with primary antibody (1:200) overnight, washed 3x with PBS, probed with tubulin (Alexa Fluor 594) antibody for 2 h, RT. Washed 3x with PBS, mounted on slides using prolong gold, imaged using Nikon confocal microscope (100x oil). Image from verified customer review.\" > Genetic Strategies Validation. Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - MUS81 inhibition in BRCA2-deficient cells causes accumulation of 53BP1 nuclear bodies and G1 arrest. H1299 cells carrying a DOX-inducible BRCA2 shRNA were transfected with control or MUS81 siRNAs. Representative images of cells processed 72 h later for immunostaining with 53BP1 Antibody (green) and anti-cyclin A (red) antibodies. DNA was counterstained with DAPI. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms15983), licensed under a CC-BY licence.\" > Biological Strategies Validation. Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Pot1a prevents DDR in HSCs. Telomeric DDR in 8 week-old LSK cells upon Pot1a knockdown. Immunocytochemical staining of TRF1 (green). Foci co-stained with TRF1 and 53BP1 were identified as TIFs. Nuclei were stained with TOTO3 (blue). Scale bar, 2 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00935-4), licensed under a CC-BY licence.\" > Reactivity:Hu, Mu, Rt, Bt, Bv, Ca, Fi, Gt, Pm, Po, Pm, Fe, Pm-Or, Pm, Rb, ShSpecies GlossaryReactivity:Hu, Mu, Rt, Bt, Bv, Ca, Fi, Gt, Pm, Po, Pm, Fe, Pm-Or, Pm, Rb, ShApplications:WB, ChIP, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, KD, KOApplications:WB, ChIP, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, KD, KOHost:Rabbit PolyclonalHost:Rabbit PolyclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Validated by:Validated by: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB600-501beta-Actin Antibody (AC-15)Purity: UnpurifiedImmunogen: This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.Other Names: ACTB, actin, beta, actin, cytoplasmic 1, anti-beta actin, B-Actin, Beta Actin, beta actin (ac-15), beta cytoskeletal actin, Beta-actin, beta-actin ac 15, beta-actin ac15, beta-actin ac-15, BRWS1, I(2)-Actin, PS1TP5-binding protein 1, PS1TP5BP1\"> Reactivity:Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Tr, Ze, Dr(-)Species GlossaryReactivity:Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Tr, Ze, Dr(-)Applications:WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KOApplications:WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KOHost:Mouse MonoclonalHost:Mouse MonoclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB100-479HIF-1 alpha AntibodyPurity: Immunogen affinity purifiedImmunogen: This HIF-1 alpha antibody was developed against a fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221].Other Names: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1, PAS domain-containing protein 8, PASD8\"> Biological Strategies Validation. Chromatin Immunoprecipitation: HIF-1 alpha Antibody [NB100-479] - ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine.\" > Biological Strategies Validation. Western Blot: HIF-1 alpha Antibody [NB100-479] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type\'s negative (non-irradiated) control and the means & SEMs are plotted. Each result represents at least 3 independent samples, plated in triplicate. Holm-Sidak comparisons for multiple groups were used for statistical comparisons of raw values (*p Biological Strategies Validation. Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis of HIF-1 alpha in mouse hepatocytes (left lane: normoxic and right lane: hypoxic) using HIF-1 alpha antibody. Western blot image submitted by a verified customer review.\" > Biological Strategies Validation. Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.\" > Biological Strategies Validation. Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.\" > Reactivity:Hu, Mu, Rt, Po, Ca, Fi, Gt, Ha, Pm, RbSpecies GlossaryReactivity:Hu, Mu, Rt, Po, Ca, Fi, Gt, Ha, Pm, RbApplications:WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-WhMtApplications:WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-WhMtHost:Rabbit PolyclonalHost:Rabbit PolyclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Validated by:Validated by: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB400-105ABCA1 AntibodyPurity: Immunogen affinity purifiedImmunogen: Partial peptide sequence (within residues 1100-1300) of human ABCA1 Antibody [UniProt# O95477]. Actual immunogen sequence is proprietary information.Other Names: ABC-1, ABC1ATP-binding cassette transporter A1, ATP-binding cassette 1, ATP-binding cassette transporter 1, ATP-binding cassette, sub-family A (ABC1), member 1, CERP, Cholesterol efflux regulatory protein, EC 2.7.7.8, EC 2.8.1.8, EC 3.6.3, EC 3.6.3.41, FLJ14958, HDLDT1, membrane-bound, MGC164864, MGC165011, TGDATP-binding cassette sub-family A member 1\"> Orthogonal Strategies Validation. Dual RNAscope ISH-IHC: ABCA1 Antibody [NB400-105] - Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.\" > Biological Strategies Validation and Orthogonal Strategies Validation.Western Blot: ABCA1 Antibody [NB400-105] - Analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total cell post-nuclear lysate from each group.\" > Biological Strategies Validation. Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [NB400-105] - HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.\" > Biological Strategies Validation. Western Blot: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate.\" > Reactivity:Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, RbSpecies GlossaryReactivity:Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, RbApplications:WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, Dual ISH-IHC, Func, GS, KD, KOApplications:WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, Dual ISH-IHC, Func, GS, KD, KOHost:Rabbit PolyclonalHost:Rabbit PolyclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Validated by:Validated by: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB500-201Survivin AntibodyPurity: Immunogen affinity purifiedImmunogen: This Survivin Antibody was developed against full length recombinant human Survivin [UniProt# O15392]Other Names: API4, apoptosis inhibitor 4, Apoptosis inhibitor survivin, baculoviral IAP repeat-containing 5, Baculoviral IAP Repeat-Containing Protein 5, BIRC5, EPR-1, IAP4, survivin variant 3 alpha\"> Orthogonal Strategies Validation. Dual RNAscope ISH-IHC: Survivin Antibody [NB500-201] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.\" > Orthogonal Strategies Validation. Western Blot: Survivin Antibody [NB500-201] - Analysis of 30ug of HeLa whole cell lysate [NB800-PC1] using rabbit polyclonal [NB500-201] at 1ug/ml. Detection was performed using ECL method with 1 minute exposure. Band detected at higher molecular weight than the predicted MW (16 kDa).\" > Genetic Strategies Validation. Knockdown Validated: Survivin Antibody [NB500-201] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the tumor tissues on day 19 using [NB500-201]. Scale bar, 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116022) licensed under a CC-BY licence.\" > Biological Strategies Validation. Knockdown Validated: Survivin Antibody [NB500-201] - Aurora B phosphorylates EB2. (c,d) HeLa cells were transfected with siRNAs as indicated; after 24 h, the cells were treated with nocodazole for 4 h. Mitotic cells were harvested by mitotic shake-off. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11117), licensed under a CC-BY licence.\" > Reactivity:Hu, Mu, Rt, Ca, Fe, Gp, HaSpecies GlossaryReactivity:Hu, Mu, Rt, Ca, Fe, Gp, HaApplications:WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Dual ISH-IHC, KDApplications:WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Dual ISH-IHC, KDHost:Rabbit PolyclonalHost:Rabbit PolyclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Validated by:Validated by: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB300-221GAPDH Antibody (1D4)Purity: Immunogen affinity purifiedImmunogen: This GAPDH antibody was developed against full length purified porcine GAPDH [UniProt# P00355]Other Names: 1D4 GAPDH, aging-associated gene 9 protein, Cytoplasm Marker, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, GAPDPeptidyl-cysteine S-nitrosylase GAPDH, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38, OCT1 coactivator in S phase, Peptidyl-cysteine S-nitrosylase GAPDH\"> Reactivity:Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, ZeSpecies GlossaryReactivity:Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, ZeApplications:WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single-Cell WesternApplications:WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single-Cell WesternHost:Mouse MonoclonalHost:Mouse MonoclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:Unconjugated \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB600-1384LC3B AntibodyPurity: Immunogen affinity purifiedImmunogen: Polyclonal LC3B Antibody was made to a synthetic peptide made to the N-terminal region of the human LC3B protein. [Uniprot: Q9GZQ8]Other Names: ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B\"> Biological Strategies Validation. Western Blot: LC3B Antibody [NB600-1384] - Analysis of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using anti- [Catalog # NB600-1384].\" > Biological Strategies Validation. Immunohistochemistry: LC3B Antibody [NB600-1384] - Staining of treated U373-MG (human glioblastoma) cells using anti- [Catalog # NB600-1384].\" > Biological Strategies Validation. Immunocytochemistry/Immunofluorescence: LC3B Antibody [NB600-1384] - Immunocytochemical/Immunofluorescent staining of treated U373-MG cells using the HRP conjugate of anti- (Catalog # NB600-1384). The nuclei were stained with DAPI.\" > Biological Strategies Validation. Western Blot: LC3B Antibody [NB600-1384] - Depletion of RARalpha prevents ATRA-induced autophagic flux in SKBR3 cells. LC3B western blotting and quantification of the autophagic activity in control and RARalpha-knockdown SKBR3 cells upon treatment with 1 uM ATRA for 2 days in the presence or absence of BafA for 2 h at 200 nM. LC3B-II expression was normalized to GAPDH and to vehicle-treated control cells. Standard deviations from at least five independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2015236), licensed under a CC-BY licence.\" > Reactivity:Hu, Mu, Rt, Po, Ba, Bv, Ca, Pm, ZeSpecies GlossaryReactivity:Hu, Mu, Rt, Po, Ba, Bv, Ca, Pm, ZeApplications:WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, KD, KOApplications:WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, KD, KOHost:Rabbit PolyclonalHost:Rabbit PolyclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Validated by:Validated by: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NB100-2331LC3A AntibodyPurity: Immunogen affinity purifiedImmunogen: This LC3A Antibody was prepared from a synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492].Other Names: ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, Microtubule-associated protein 1 light chain 3 alpha, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A\"> Reactivity:Hu, Mu, Rt, Am, Ca, Fi, Pl, Ze, BvSpecies GlossaryReactivity:Hu, Mu, Rt, Am, Ca, Fi, Pl, Ze, BvApplications:WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SB, IHC-WhMtApplications:WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SB, IHC-WhMtHost:Rabbit PolyclonalHost:Rabbit PolyclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) NBP2-46892LC3B Antibody (1251A)Purity: Protein G purifiedImmunogen: Recombinant monoclonal LC3B Antibody (1251A) was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8].Other Names: ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B monoclonal, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B\"> Genetic Strategies Validation. Knockout Validated: LC3B Antibody (1251A) [NBP2-46892] - Western Blot analysis shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 2.0 ug/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (1251A) [Catalog # NBP2-46892] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.\" > Genetic Strategies Validation. Knockout Validated: LC3B Antibody (1251A) [NBP2-46892] - LC3B was detected in immersion fixed Chloroquine treated Hela cells (left) but was not detected in LC3B knockout Hela cells (right) using rabbit anti-human LC3B monoclonal antibody (1251A) [Catalog #NBP2-46892] at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.\" > Biological Strategies Validation. Flow (Intracellular): LC3B Antibody (1251A) [NBP2-46892] - Jurkat cells were either untreated (A) or treated with 50uM chloroquine for 24 hours (B). An intracellular stain was performed with anti-LC3B (1251A) antibody [Catalog # NBP2-46892] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).\" > Biological Strategies Validation. Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Analysis shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with Chloroquine. PVDF membrane was probed with 0.2 ug/mL rabbit anti-LC3B monoclonal Antibody (1251A) (Catalog # NBP2-46892, Novus Biologicals), followed by 1:2000 dilution of goat anti-rabbit IgG secondary antibody. A specific band was detected for LC3B at a molecular weight of approximately 15 kDa in CQ treated NIH-3T3 and PC12 cell lines.\" > Biological Strategies Validation. Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot analysis using HeLa cells treated with (+) or without (-) Chloriquine (CQ). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa.\" > Biological Strategies Validation. Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot image of monoclonal anti-LC3B Antibody (Clone 1251D) [Catalog # NBP2-46892]. HeLa and Neuro2A cells were treated with or without 50 uM chloroquine for 24 hours as indicated. Whole cell protein was then separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 min and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2 ug/ml anti-LC3B Antibody in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the accumulation of LC3B II upon chloroquine treatment. Bands for LC3 were detected at a molecular weight of approximately 15 kDa in treated HeLA cells, and both treated and untreated Neuro2A cells.\" > Reactivity:Hu, Mu, Rt, Po, Bt, Bv, Fi, Xp, ZeSpecies GlossaryReactivity:Hu, Mu, Rt, Po, Bt, Bv, Fi, Xp, ZeApplications:WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KOApplications:WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KOHost:Rabbit MonoclonalHost:Rabbit MonoclonalSizes:Sample Size AvailableSizes:Sample Size AvailableConjugate:UnconjugatedAvailable Conjugates: Validated by:Validated by: Conjugates \",\"Price\":\"Price Not Shown\"}\'>compare (select up to 3 products from this page to compare) page of 4395